Clinical symptoms and signs in hamsters during experimental infection with the SARS-CoV-2 virus (Coronaviridae: <i>Betacoronavirus</i>)

Introduction. At the beginning of December 2019, humanity has faced a new problem caused by coronavirus. In Hubei province of central China, epidemic events associated with severe primary viral pneumonia in humans began to develop. The isolated etiological agent was identified as a representative of Coronaviridae family. The global pandemic associated with the new coronavirus infection, acute respiratory syndrome type 2 (Severe acute respiratory syndrome 2, SARS-CoV-2), has become a challenge for humanity. Objective. In our work, we assessed the replicative ability and pathogenesis of the SARS-CoV-2 virus in hamsters. Materials and methods. Syrian hamsters (n=16) randomly divided into two groups were used in experiment. The first group was infected intranasally with the SARS-CoV-2 virus, strain SARS-CoV-2/human/KAZ/KZ_Almaty/2020 deposited in GenBank under number MZ379258.1. The second group remained as a control group. Clinical manifestations of the disease in hamsters were observed within 14 days. Samples were collected on days 3, 5, 7, 9, 12, and 14 postinfection. The obtained samples were tested for viral isolation in cell culture, histological examination and analysis of viral RNA by RT-PCR. Results. SARS-CoV-2 virus isolates showed efficient replication in the lungs of hamsters, causing pathological lung lesions in animals infected intranasally. Clinical manifestations of the disease in hamsters infected with this virus were characterized by a decrease in temperature and body weight, wetness and ruffled fur, and frequent stroking of the nasal planum. High virus titers were observed following the virus isolation in cell cultures from nasal, oral swabs and lungs of animals infected intranasally. Pathological autopsy demonstrated pathological changes in the lungs. Moreover, transmission by airborne droplets has been established when a healthy hamster was kept together with animals infected using the intranasal method. Conclusion. In conclusion, our study showed that the Syrian hamster model is a useful tool for studying the SARS-CoV-2 pathogenesis, as well as testing vaccine candidates against acute respiratory syndrome type 2

Human genome meeting 2016 : Houston, TX, USA. 28 February - 2 March 2016

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Ouyang P1 A meta-analysis of genome-wide association studies of mitochondrial dna copy number A. Moore, Z. Wang, J. Hofmann, M. Purdue, R. Stolzenberg-Solomon, S. Weinstein, D. Albanes, C.-S. Liu, W.-L. Cheng, T.-T. Lin, Q. Lan, N. Rothman, S. Berndt P2 Missense polymorphic genetic combinations underlying down syndrome susceptibility E. S. Chen P4 The evaluation of alteration of ELAM-1 expression in the endometriosis patients H. Bahrami, A. Khoshzaban, S. Heidari Keshal P5 Obesity and the incidence of apolipoprotein E polymorphisms in an assorted population from Saudi Arabia population K. K. R. Alharbi P6 Genome-associated personalized antithrombotical therapy for patients with high risk of thrombosis and bleeding M. Zhalbinova, A. Akilzhanova, S. Rakhimova, M. Bekbosynova, S. Myrzakhmetova P7 Frequency of Xmn1 polymorphism among sickle cell carrier cases in UAE population M. 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Ucar P54 Direct enrichment for the rapid preparation of targeted NGS libraries C. L. Hendrickson, A. Emerman, D. Kraushaar, S. Bowman, N. Henig, T. Davis, S. Russello, K. Patel P56 Performance of the Agilent D5000 and High Sensitivity D5000 ScreenTape assays for the Agilent 4200 Tapestation System R. Nitsche, L. Prieto-Lafuente P57 ClinVar: a multi-source archive for variant interpretation M. Landrum, J. Lee, W. Rubinstein, D. Maglott P59 Association of functional variants and protein physical interactions of human MUTY homolog linked with familial adenomatous polyposis and colorectal cancer syndrome Z. Abduljaleel, W. Khan, F. A. Al-Allaf, M. Athar , M. M. Taher, N. Shahzad P60 Modification of the microbiom constitution in the gut using chicken IgY antibodies resulted in a reduction of acute graft-versus-host disease after experimental bone marrow transplantation A. Bouazzaoui, E. Huber, A. Dan, F. A. Al-Allaf, W. Herr, G. Sprotte, J. Köstler, A. Hiergeist, A. Gessner, R. Andreesen, E. Holler P61 Compound heterozygous mutation in the LDLR gene in Saudi patients suffering severe hypercholesterolemia F. Al-Allaf, A. Alashwal, Z. Abduljaleel, M. Taher, A. Bouazzaoui, H. Abalkhail, A. Al-Allaf, R. Bamardadh, M. Atha

Қазіргі жағдайдағы Қазақстанның шағын және орта кәсіпкерлігінің даму ерекшеліктері және проблемалары

Шағын және орта бизнестің дамуы нарықтық экономиканың табысты дамуының кепілі. Халықаралық тәжірибе көрсеткендей, шағын және орта бизнестің ойдағыдай дамуы мен жұмыс істеуі үшін тиімді құқықтық базаны құру, мемлекет тарапынан тиісті құқықтық және қаржылық қолдаумен қамтамасыз ету қажет. Мақаланың мақсаты – Қазақстандағы шағын және орта кәсіпкерліктің дамуына әсер ететін факторларды анықтау, шағын және орта кәсіпкерліктің сипаттамаларын, даму тенденциялары мен проблемаларын талдау, сондай-ақ әлемдік экономиканың қазіргі дағдарыс жағдайында шағын және орта кәсіпкерліктің тиімділігі мен қаржыландырылуын арттыру бойынша ұсыныстар әзірлеу. Соңғы 10 жылдағы ұлттық экономиканың әртүрлі салаларындағы шағын және орта бизнестің қызметінің динамикасы талданып, оның дамуына әсер ететін негізгі факторлар, өзекті проблемалар мен қазіргі тенденциялар анықталды. Зерттеу барысында диалектиканың әр түрлі әдістері қолданылды: талдау, жалпылау, синтез, индукция, дедукция. 2010-2020 жылдар арасындағы Қазақстан Республикасындағы шағын және орта кәсіпорындардың саны туралы статистикалық мәліметтер жинақталып, SWOT талдау әдісін қолдана отырып қорытындылар жасалды. COVID-19 пандемиясының 2020 жылы наурызда отандық экономикаға енген кезінен бастап Қазақстандағы шағын және орта бизнестің дамуына әсері қарастырылды. Зерттеу барысында шағын және орта кәсіпкерліктің тиімділігін төмендететін келесі негізгі проблемалар анықталды, мысалы: ғылым мен өндіріс арасындағы әлсіз байланыстар, бюрократия, әкімшілік және мемлекеттік құрылымдардың икемсіздігі, шағын және орта бизнесті мемлекеттік қаржыландыру тетіктерінің жеткіліксіз тиімділігі. Авторлар пандемия мен қазіргі жағдайда шағын және орта бизнестің тиімділігін арттыру, сондай-ақ Қазақстандағы кәсіпкерлікті мемлекеттік қолдау бойынша ұсыныстар береді

Influence of Gamma-glutamyl carboxylage (GGCX) on warfarin dose

Warfarin is an oral anticoagulant which is popular in cardiology for the prevention and treatment of thromboembolic events in patients with deep vein thrombosis. Patients usually treated according to the clinical data. However, incorrect dose of the treatment can be followed with side effects such as excessive bleeding and thrombosis. To avoid these side effects patients need genetic tests for specific genes which are responsible for the sensitivity of patient to warfarin. GGCX is one of the genes which have a role in dose of the warfari

Influence of Gamma-glutamyl carboxylage (GGCX) on warfarin dose

Warfarin is an oral anticoagulant which is popular in cardiology for the prevention and treatment of thromboembolic events in patients with deep vein thrombosis. Patients usually treated according to the clinical data. However, incorrect dose of the treatment can be followed with side effects such as excessive bleeding and thrombosis. To avoid these side effects patients need genetic tests for specific genes which are responsible for the sensitivity of patient to warfarin. GGCX is one of the genes which have a role in dose of the warfari

Mutual Interaction Between Literature and Architectural Space

Examples of the interaction of literature and architectural space are considered in this article. The considered examples demonstrate the role of literature in solving various architectural problems. For example, social tasks related to the safety of the urban environment, in the creation of a unique image of the city, the development of stylistic trends, environmental problems. Human perception of architectural space, described in the literature, can make an invaluable contribution to scientific research in the social and humanitarian direction. The description of architectural spaces allows us to identify the level of comfort, the influence of architectural objects and landscape on people, the correspondence of the urban environment to the worldview of the townspeople. Both directions have huge scientific potential in research activities. The result of this research is the interpretation of the literary description of the subject environment of applied folk art in a modern interior. The architectural space has a scenic character reflecting the literary descriptions of the yurt's interior. This approach can be used as the main direction in the development of the concept of architectural spaces for different functional purposes. This article examines an example of a kindergarten organized according to the principle of "the walls teach"